قسم الأحياء الدقيقة والطفيليات

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حول قسم الأحياء الدقيقة والطفيليات

من يعمل بـقسم الأحياء الدقيقة والطفيليات

يوجد بـقسم الأحياء الدقيقة والطفيليات أكثر من 13 عضو هيئة تدريس

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د. عبدالقادر الضاوي الفيتوري الضاوي

عبدالقادر هو احد اعضاء هيئة التدريس بقسم الاحياء الدقيقة والطفليات بكلية الطب البيطري. يعمل السيد عبدالقادر بجامعة طرابلس كـاستاذ مساعد منذ 2017-01-01 وله العديد من المنشورات العلمية في مجال تخصصه

منشورات مختارة

بعض المنشورات التي تم نشرها في قسم الأحياء الدقيقة والطفيليات

Identification of phenolic compounds, antibacterial and antioxidant activities of raisin extracts

In this study, antibacterial, antioxidant and phenolic compounds of raisin extracts was evaluated. Different solvent extraction methods were utilized to extract phenolic compounds from raisin. The phenolic compounds of raisin extracts was determined using HPLC and three compounds were found, catechin, quercetin and rutin. The antibacterial activity of the acetonic extract was tested against four bacterial pathogens viz. Staphylococcus aureus, Pseudomonas aeroginosa, Salmonella typhimurium and Escherichia coli using both disk diffusion and minimum inhibitory concentration (MIC) methods. The acetonic extract exhibited the highest activity against Staphylococcus aureus with zone of inhibition of 14mm and the MIC of 25 mg/ml. However, there was a little activity against E. coli and S. typhimurium. The methanolic extract showed good antioxidant activity as indicated by 2,2-diphenyl-1-picrylhydrazyl free radical assay (DPPH). In conclusion, raisin contains potent phenolic compounds and their application might function as promising natural preservative and antimicrobial agents in food industry and also good antioxidant food that helps to minimize the risk of degenerative diseases. arabic 11 English 86
Abdulgader Dhawi Alfitouri Dhawi(1-2021)
Publisher's website

Salmonella Enteritidis’ Proteins produce in Vitro and in Vivo Protection against Colonization

Salmonella enterica can be considered as one of the most important causes of foodpoisoning with poultry thought to be the main source. Although S. Typhimurium, S. Enteritidis and the vast majority of other Salmonella serovars generally produce little systemic disease in adult chickens, they are able to colonize the alimentary tract of poultry. The two caeca are the main sites of the colonization of Salmonellae in chickens, and the bacteria can be easily harvested from the caeca for analysis. Bacterial proteins analysed utilizing SDS-PAGE showed differences between in vitro and in vivo that out of about 40 protein bands of in vitro preparation only a few (3-5) bands can be visualized from in vivo preparations. We suggested that some avian proteases might be responsible. Accordingly, and to investigate the hypothesis that bacterial-precipitated protein harvested from chickens is thought to be more protective than bacteria grown in broth culture, the immunogenicity of protein-precipitated vaccines harvested from chicken intestine and those from broth culture (in vitro), were compared using bacterial proteins as an orally inoculated vaccine candidate in chicken. The results demonstrated that the in vitro sonicated proteins obtained from a nutrient broth culture had a much better protective vaccine effect than the in vivo sonicated proteins preparations harvested from bacteria grown in chickens arabic 14 English 81
Altayeb Elazomi, Elhadi Araibi, Abdulgader Dhawi, Hatem Khpiza, Susan Liddell, Margret Lovell, Paul Barrow(12-2016)
Publisher's website

Bovine digital dermatitis and severe virulent ovine foot rot: a common spirochaetal pathogenesis

A potential pathological role for spirochaetes in bovine digital dermatitis (bovine DD) and severe virulent ovine foot rot (SVOFR) has been considered and a treponeme isolate obtained from each disease in the UK. In this work, we have investigated the hypothesis that the two diseases may have a shared (common) spirochaetal aetiology. Experiments were designed to identify serological similarities and differences between the two spirochaetes; an enzyme-linked immunosorbent assay (ELISA) was developed to detect anti-treponeme antibodies in the sera of cows and sheep against the two-treponeme isolates. Sera were further tested for antigen reactivity by Western blotting. Cattle and sheep with bovine DD and SVOFR, respectively, had increased seropositivity rates to both treponeme isolates, with different patterns of reactivity between farms. In some cattle herds, significant correlations were shown between antibodies to bovine DD treponemes and SVOFR treponemes (P0.05); sheep showed strong evidence of reactivity to one or the other treponeme antigens, but never to both. Western blotting against both treponeme antigens showed that they frequently displayed different antigen epitopes, although some minor bands were common to both organisms. The data suggest that there are a number of spirochaetes in UK farms, which could be involved in the pathogenesis of either bovine DD or SVOFR. arabic 13 English 83
Abdulgader Dhawi Alfitouri Dhawi(1-2021)
Publisher's website