المنشورات العلمية لـكلية الطب البيطري

احصائيات منشورات كلية الطب البيطري

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    وثيقة

FETLOCK JOINT ANGLE MEASUREMENT IN EQUINE FORELIMBS

Front fetlock joint angle (FA) measurement might predict musculoskeletal injury. This study aimed to identify an accurate reliable tool, and develop a consistent method for measuring the FA. Fetlock centre of rotation was identified radiographically. Goniometer measurements were validated on ten vertically fixed post-mortem distal forelimbs by positioning the centre of rotation of the goniometer on the approximate centre of rotation of the joint for five measurements of each FA at three day intervals and one measurement by another person (rater B). Both FA’s of fourteen sound horses standing squarely on a concrete floor were repeated before and after lifting the contralateral limb, which was flexed at the carpus and held at the same level as the carpus of the standing limb. Consistency of measurements was assessed using both fetlock joints of five horses standing squarely on a concrete floor measured six times in random order and then with the contralateral limb lifted using both front fetlocks of eight horses measured five times in random order. The repeatability coefficient was calculated with WinPepi v10.5 software. The centre of rotation was on or around the eminence of the lateral collateral ligament. The repeatability coefficient of the goniometer validation was 4.7°. The mean FA for rater A and B were not significantly different (161.4° versus 162.9°). FA’s were significantly less after lifting the contralateral limbs (P
Abdulrhman M. Alrtib, Helen M S Davies(7-2012)

Successful Anatomical Access for Surgical Removing of the Distal Fracture of Splint Bones in Thoroughbred Horses (Case Study)

Poor performance in athletic horses due to musculoskeletal affections is very common in Libya. Two mature thoroughbred horses suffering from poor performance and history of moderate degree forelimb lameness were admitted to the Aswany Private Equine clinic in Tripoli, Libya. The study is aiming for presenting a simple, time effective, cost effective surgical procedure to treat distal simple splint bone fracture in thoroughbred horses. Horses were examined thoroughly for the common muscle, hoof, and tendons affections. A progressed unilateral left forelimb swelling involving the lateral distal part of the fourth metacarpal bones was noticed. Three radiographs were taken to each horse including latero- medial, dorsolateral-palmaromedial, and palmarolateral-dorsomedial views to assess both splint bones. The radiographs showed that there is a complete fracture involving the distal extremity (button) of the fourth metacarpal bones. Surgical excisions of the fractured part of the splint bones have been decided to treat these horses. To precisely locate the site of the bone fragments, 1.5 mm stainless steel wires were placed over the distal part of splint prior to taking the radiographs. The surgical sites were aseptically prepared, and the horses were then sedated via intravenous injection of xylazine at a dose rate of 1 mg/kg body weight. The site of surgical incision was infiltrated with 2% lidocaine. The bone fragments were then removed after performing the surgical incision. The sharp end at the distal end of the proximal part of the splint bone was then smoothening with a scissor. The surgical incisions were then routinely closed and the post-operative care and radiographs were taken. The surgical incision made complete healing within two weeks. Both horses were followed up for up to three years post-operation, and they did well compared to what has been done in other studies. We concluded that the surgical technique used to treat the two horses with splint bone fracture enrolled in the current study is a simple and effective procedure. arabic 19 English 106
Mohamed H. Abushhiwa, Osma K Sawesi, Khaled A. Milad, Aiman. A. Shalgum, Abdulrhman Mohamed Salah Alrtib(9-2019)

Vancomycin-Resistant Enterococci: A Review of Antimicrobial Resistance Mechanisms and Perspectives of Human and Animal Health

Vancomycin-resistant enterococci (VRE) are both of medical and public health importance associated with serious multidrug-resistant infections and persistent colonization. Enterococci are opportunistic environmental inhabitants with a remarkable adaptive capacity to evolve and transmit antimicrobial-resistant determinants. The VRE gene operons show distinct genetic variability and apparently continued evolution leading to a variety of antimicrobial resistance phenotypes and various environmental and livestock reservoirs for the most common van genes. Such complex diversity renders a number of important therapeutic options including "last resort antibiotics" ineffective and poses a particular challenge for clinical management. Enterococci resistance to glycopeptides and multidrug resistance warrants attention and continuous monitoring.
Mohamed O. Ahmed , Keith E Baptiste (6-2017)
موقع المنشور

Colistin-resistant carbapenemase-producing isolates among Klebsiella spp. and Acinetobacter baumannii in Tripoli, Libya

The emergence of acquired carbapenemases is a serious threat to public health worldwide, forcing the use of last-resort antibiotics such as polymyxins. Use of such molecules had recently led to the emergence of colistin-resistant carbapenemase-producing isolates, leaving only a few therapeutic options for the near future. Here we report the identification of colistin-resistant isolates among a collection carbapenemase-producing Enter- obacteriaceae and Acinetobacter baumannii isolates.
Nicolas Kieffer , Ahmed MO et al.(6-2018)
موقع المنشور

Identification of phenolic compounds, antibacterial and antioxidant activities of raisin extracts

In this study, antibacterial, antioxidant and phenolic compounds of raisin extracts was evaluated. Different solvent extraction methods were utilized to extract phenolic compounds from raisin. The phenolic compounds of raisin extracts was determined using HPLC and three compounds were found, catechin, quercetin and rutin. The antibacterial activity of the acetonic extract was tested against four bacterial pathogens viz. Staphylococcus aureus, Pseudomonas aeroginosa, Salmonella typhimurium and Escherichia coli using both disk diffusion and minimum inhibitory concentration (MIC) methods. The acetonic extract exhibited the highest activity against Staphylococcus aureus with zone of inhibition of 14mm and the MIC of 25 mg/ml. However, there was a little activity against E. coli and S. typhimurium. The methanolic extract showed good antioxidant activity as indicated by 2,2-diphenyl-1-picrylhydrazyl free radical assay (DPPH). In conclusion, raisin contains potent phenolic compounds and their application might function as promising natural preservative and antimicrobial agents in food industry and also good antioxidant food that helps to minimize the risk of degenerative diseases. arabic
Yousef M Abouzeed, Ahmed MO Et Al. (12-2018)
موقع المنشور

An evolving research culture: Analysis of biomedical publications from Libya, 2003–13

Understanding the publication output of a country’s biomedical research can provide information for strengthening its policies, economy, and educational systems. Yet, this is the first bibliometric study to date to analyze and provide an in-depth discussion of the biomedical research productivity from Libyan academic institutions. The biomedical research productivity of higher education institutes and affiliated hospitals from Libya, with a focus on the higher education sector, was analyzed and discussed for the period of 2003–13 using the PubMed database. A questionnaire online survey was also developed to obtain opinions of Libyan scientists on productivity status and quality of research output. A total of 345 peer-reviewed articles were included in the analysis and were authored by researchers in the cities of Benghazi (32%), Tripoli (29%), Al-Zawiya (9%), Al-Bayda (8%), Sabha (3%), Misrata (3%), and Al-Khoms (1%). The papers included co-authors and/or corresponding authors from over 40 other countries were published in 179 journals covering a broad range of biomedical topics. Questionnaire (44% response rate) indicated research is largely self-funded. Information along with accurate, comprehensive, and transparent metrics can be applied to aid governmental and nongovernmental institutions to develop stable infrastructures for academic communities to achieve effective research performance and innovation.
Mohamed O. Ahmed, Et Al.(10-2017)
موقع المنشور

Analysis of biomedical publications in Libya from 2003 to 2013

A relatively low scientific research output is not unique to Libya and unfortunately persists as compared to more economically developed countries.[1],[2] Recent data have revealed the very low-productivity rate of research from Libya, compared to other Arab and North African nations.[1],[3],[4] In 2003, Libya's hierarchal ranking among 20 Arabic countries was 12th for annual publication rate, 10th for publication rate according to population, and 15th for publication rate according to Gross Domestic Product (GDP).[5] As Libya undergoes geopolitical and socioeconomic upheavals, there is certainly a unique opportunity for improving all aspects of its educational and economic infrastructures. The post-2011 era and the current civil upheaval have, however, resulted in disrupted infrastructure, social incoherence, and a fragmented society, affecting every aspect of daily life of its citizens. Libya's research output was investigated by analyzing the collective peer-reviewed publications of biomedical research from Libya, with a focus on institutions of higher education and the medical sector revealing important knowledge and novel scientometric data.[1]
Mohamed O. Ahmed, Et Al.(5-2019)
موقع المنشور

Whole Genome Sequence Analysis of the First Vancomycin-Resistant Enterococcus faecium Isolates from a Libyan Hospital in Tripoli

Abstract The purpose of the study was to investigate the molecular characteristics and genetic relatedness of the first reported cases of vancomycin-resistant enterococci (VRE) from the Tripoli Medical Center, Libya. In total, 43 VRE isolates were obtained from various clinical sites throughout the years 2013-2014, including 40 vanA-type and 2 vanB-type vancomycin-resistant Enterococcus faecium isolates and 1 vanC1-type Enterococcus gallinarum. Of the 42 E. faecium, 19 isolates were subjected to whole genome sequencing. Core genome multilocus sequence typing (cgMLST) analysis revealed three sequence clusters (SCs) of clonally related isolates, which were linked to different hospital wards. The first two VRE isolates, isolated early 2013 from patients in the medical intensive care unit, were grouped in SC1 (MLST [ST] 78, vanB) and differed in only 3 of 1423 cgMLST alleles. The SC2 (n = 16, special care baby unit, neonatal intensive care unit, pediatric surgery ward, and oncology ward) and SC3 (n = 1, antenatal ward) were all ST80 vanA-VRE, but the single SC3 isolate differed in 233 alleles compared with SC2. Within SC2, isolates differed in 1-23 alleles. Comparison with a larger database of E. faecium strains indicated that all isolates clustered within the previously defined hospital clade A1. A combination of Resfinder and mlplasmid analysis identified the presence of resistance genes on different plasmid predicted genetic elements among different SCs. In conclusion, this study documents the first isolates causing outbreaks with VRE in the Libyan health care system. Further surveillance efforts using molecular typing methods to monitor spread of multidrug-resistant bacteria in the Libyan health care system are urgently needed.
Mohamed O. Ahmed, Et Al.(10-2020)
موقع المنشور