المنشورات العلمية لـقسم أمراض الدواجن والأسماك

احصائيات منشورات قسم أمراض الدواجن والأسماك

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    وثيقة

Seroprevalence and molecular detection of Newcastle disease virus in backyard chickens in Tripoli, Libya

Abstract Background: Newcastle disease (ND) is a viral disease that affecting many avian species all over the world. Aim: ND has been successfully controlled by the vaccination of commercial poultry in Libya. However, there was a lack of information about the situation of ND in backyard chickens. Therefore, this study determined the prevalence of ND in backyard chickens in different locations of Tripoli. Methods: A total number of 280 cloacal swabs (190 in summer and 90 in winter) and 412 sera were collected from non-vaccinated backyard chicken flocks in different geographical locations within the area of Tripoli namely Qasr Ben Ghashier, Al-Sawani, Souq Al-Gomaa, Tajourah, Ein Zara, and Janzour. Cloacal swabs and sera were tested by real time polymerase chain reaction (PCR) and ELISA, respectively. Results: The prevalence of ND virus (NDV) infection in backyard chickens in different locations of Tripoli during summer and winter was 45% using real-time reverse transcription-PCR. Except in Qasr Ben Ghashier, the prevalence in summer season was significantly higher than in winter (X2 = 46.13, p ≥ 0.00001). ELISA test revealed 218 positive out of 412 tested samples with total prevalence of 53% across the city of Tripoli in all regions. Obviously, Qasr Ben Ghashier had significantly (X2 = 74.09, p ≥ 0.00001) the highest prevalence (82%) of NDV specific antibodies followed by Tajourah (68%). Conclusion: This study demonstrated the situation of ND in backyard chicken highlighting the necessity of a comprehensive vaccination plan for backyard chickens. arabic 14 English 90
ِAbdulwahab Kammon, Emhemed A. Gedara, Hesham A. Alseed, Abdunaser S. Dayhum, Mohamed A. Benothman, Soleman O. Al-Garib, Ibrahim M. Eldaghayes(3-2020)
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Isolation, Serological and Molecular Detection of Infectious Laryngotracheitis Virus (ILTV) in Chickens in Libya

Infectious Laryngotracheitis (ILT) is a viral respiratory disease of poultry caused by Gallid herpesvirus 1 leading to severe economic losses. The aim of the current study was to isolate and identify ILT virus in suspected outbreaks of one broiler flock (farm 1) and three layer flocks (farms 2,3 and 4) located in Tripoli. Samples of trachea were collected and preserved in transport media for viral isolation and some were preserved in 10% neutral formalin for histopathology. Broiler and Layer flocks were serologically monitored and tested for the presence of antibodies by ELISA against ILTV at first days old and later at slaughter age for broilers and before the onset of production for layers in which total of 290 blood samples were collected from six poultry farms (3 broiler flocks and 3 layer flocks). Following isolation of the virus in fertile eggs, chorio-allantoic membrane (CAM) samples were confirmed by PCR and histopathology. The lesions seen on the CAMs were congestion, hemorrhages and pocks after 5 days post inoculation. Samples from farm 1 and 3 were positive whereas other farms were negative. Histopathology of CAMs revealed edema, congestion, haemorrhages, infiltration of heterophils and mononuclear cells, and presence of syncytial cells containing intranuclear inclusion bodies. The trachea revealed severe destruction and sloughing of the mucosa due to necrosis. The results of this study confirmed the circulation of ILTV in commercial poultry flocks and highlighted the need for more epidemiologic investigation of the disease arabic 16 English 96
Abdulwahab Kammon, Jamaal Shabba, Yousef Abouzeed, Abdulatif Asheg (2-2020)
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